Journal: Journal of Bacteriology

Article Title: Opposing roles for iron transport systems in gallium tolerance in extraintestinal pathogenic Escherichia coli

doi: 10.1128/jb.00349-25

Figure Lengend Snippet: Genes controlling fitness of M12 during growth in gallium nitrate. Transposon sequencing was used to track mutants passaged in Davis broth or Davis broth supplemented with 800 µM gallium nitrate. Each dot on the volcano plot represents a gene with log 2 -fold change (reads mapped in no gallium/gallium) on the x -axis and the −log 10 ( P value) on the y -axis. Genes with strong depletion (right) or enrichment (left) in the presence of gallium indicate loss or gain of fitness when disrupted by transposons, respectively. Orange dots indicate genes with a twofold change and P value <0.05 as determined by DESeq2, and the names of genes investigated further are indicated.

Article Snippet: Raw count tables for each gene were analyzed using the DESeq2 package in Geneious (Biomatters).

Techniques: Sequencing